Up-regulation and increased phosphorylation of protein kinase C (PKC) delta, mu and theta in the degenerating rd1 mouse retina.

Summary, in English

The rd1 mouse serves as a model for inherited photoreceptor degeneration: retinitis pigmentosa. Microarray techniques were employed to compare the transcriptomes of rd1 and congenic wild-type retinas at postnatal day 11, when degenerative processes have started but most photoreceptors are still present. Of the several genes that were differentially expressed, focus was put on those associated with the protein kinase C (PKC) signaling pathway, in particular PKCδ, μ and θ. Microarray identified these as being up-regulated in the rd1 retina, which was confirmed by QRT-PCR. Western blotting and immunostaining, using antibodies against either total or phosphorylated variants of the PKC isoforms, revealed increased expression and phosphorylation of PKCδ, μ and θ in the rd1 retina at the protein level as well. Our results suggest that these PKC isoforms are involved in rd1 degeneration.

• Författare: Seifollah Azadi; Francois Paquet-Durand; Patrik Medstrand; Theo van Veen; Per Ekström
• Avdelning/ar: Oftalmologi, Lund; Institutionen för experimentell medicinsk vetenskap; Retinal degeneration: Molekylär patologi
• Publiceringsår: 2006
• Språk: Engelska
• Sidor: 759-773
• Publikation/Tidskrift/Serie: Molecular and Cellular Neuroscience
• Volym: 31
• Issue: 4
• Länkar: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16503160&dopt=Abstract; http://dx.doi.org/10.1016/j.mcn.2006.01.001
• Dokumenttyp: Artikel i tidskrift
• Förlag: Elsevier
• Ämne: Basic Medicine; Other Clinical Medicine
• Nyckelord: Apoptosis; Kinase; Microarray; Photoreceptor; Signaling pathways
• Status: Published
• Forskningsgrupp: Retinal degeneration: Molecular Pathology
• ISBN/ISSN/Övrigt: ISSN: 1044-7431