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Nature of glomerular capillary permeability changes following acute renal ischemia/reperfusion (I/R) injury in rats.

In English

Författare

Summary, in English

This study was performed to evaluate the alterations of glomerular filtration barrier characteristics following acute renal ischemia-reperfusion (I/R). Ischemia was induced in anesthetized rats by unilateral renal artery occlusion for either 20 or 60 min, followed by reperfusion during 20 or 60 min, respectively, with the contralateral kidney serving as control. Sieving coefficients (theta) were obtained by analyzing Ficoll [mol.radius (a(e)) 13-85 angstrom] in urine and plasma after 20 and 60 min I/R. Furthermore, theta for human serum albumin (HSA) was estimated using a tissue uptake technique after 20 and 60 min of I/R, while clearance of HSA compared with that for neutralized HSA (nHSA) was assessed after 20 min of I/R only. Glomerular filtration rate (GFR) was measured by [Cr-51] EDTA and inulin. I/R reduced GFR and increased theta for Ficoll molecules of a(e) > 55 angstrom and theta for albumin. theta for Ficoll vs. a(e), analysed using a two-pore model, demonstrated that, despite increases in theta, the large-pore fractional ultrafiltration coefficient (alpha(L)) was unchanged after 20 min of I/R, owing to the decline in GFR, but increased after 60 min of I/R. However, the apparent alpha(L) for albumin increased already after 20 min of I/R (P < 0.005) and the nHSA/HSA clearance ratio was slightly reduced, possibly reflecting a diminished negative charge barrier. In conclusion, after 20 min of I/R, indications of a reduced charge selectivity were noted, while after 60 min of I/R, there was mainly a reduction in size selectivity, compatible with an increased formation of large pores.

Avdelning/ar

Publiceringsår

2006

Språk

Engelska

Sidor

1362-1368

Publikation/Tidskrift/Serie

American Journal of Physiology: Renal, Fluid and Electrolyte Physiology

Volym

291

Issue

6

Dokumenttyp

Artikel i tidskrift

Förlag

American Physiological Society

Ämne

  • Urology and Nephrology
  • Clinical Medicine

Nyckelord

  • oxidative stress
  • Ficoll
  • fractional clearance
  • albumin
  • anoxia

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 0363-6127