Du är här

Short-term culturing of teleost crystalline lenses combined with high-resolution optical measurements.

Författare:
Publiceringsår: 2010
Språk: Engelska
Sidor: 167-174
Publikation/Tidskrift/Serie: Cytotechnology
Volym: 62
Dokumenttyp: Artikel
Förlag: Springer Netherlands

Sammanfattning

Culturing whole lenses is a frequently used method for studying regulatory events on the lens in controlled environments. The evaluation methods used often fall under two categories, molecular or optical. The main benefit from optical measurements is that they directly detect changes in the lens' main function, i.e. refracting light. However, these measurements often have rather low resolution or yield results open for subjective interpretation. Here we present a short-term crystalline lens culturing technique combined with a high-resolution optical measuring method. There are two main advantages of using teleost lenses compared to mammalian lenses. Teleost tissue generally has a higher tolerance than mammalian tissue with regard to temperature and nutrient fluctuations. Teleost lenses are structurally more robust and can be excised from the eye without disturbing form or function. The technique is developed for short-term culturing (3 h), however, the lenses appear viable for at least 24 h and longer culturing may be possible. The technique is resistant to small variations in osmolarity and yields quantitative datasets for further analyses and statistical treatment.

Disputation

Nyckelord

  • Biology and Life Sciences
  • Optical measurements
  • Teleost
  • Lens
  • Organ culture

Övriga

Published
Yes
  • ISSN: 0920-9069

Box 117, 221 00 LUND
Telefon 046-222 00 00 (växel)
Telefax 046-222 47 20
lu [at] lu [dot] se

LERU logotype U21 logotype

Fakturaadress: Box 188, 221 00 LUND
Organisationsnummer: 202100-3211
Om webbplatsen