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FTIR imaging and ATR-FT-Far-IR synchrotron spectroscopy of pig ear skin

Författare:
Publiceringsår: 2010
Språk: Engelska
Sidor: 105-111
Publikation/Tidskrift/Serie: Spectroscopy: An International Journal
Volym: 24
Nummer: 1-2
Dokumenttyp: Konferensbidrag
Förlag: IOS Press

Sammanfattning

FTIR imaging was performed on pig ear skin samples cryo-sectioned perpendicular to the skin surface. The OH-stretch region revealed the distribution of water; the amide II band gave the protein distribution; the C=O stretch and C-H stretch regions showed the variation in lipids. Water and proteins were similarly distributed. Triglycerides were predominantly found in the deeper skin layers whereas free fatty acids and ceramides were more dominant in the upper layers. ATR-FT-Far-IR spectroscopy with synchrotron radiation was used on full thickness pig ear skin biopsies. The Far-IR spectra showed hands in the region from 100-150 cm(-1) due to hydrogen bonded proteins and a band around 180 cm(-1) arising from "free" water.

Disputation

Nyckelord

  • Chemistry
  • pig
  • ear skin
  • synchrotron radiation
  • FTIR imaging
  • ATR-FT-Far-IR spectroscopy

Övriga

13th European Conference on Spectroscopy of Biological Mol
AUG 28-SEP 02, 2009
Palermo, Italy
Published
Yes
  • ISSN: 0712-4813

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