Nucleoside phosphorylases from clostridium perfringens in the synthesis of 2',3'-dideoxyinosine.
Publikation/Tidskrift/Serie: Nucleosides, nucleotides & nucleic acids
Förlag: Taylor & Francis Group
Four Clostridium perfringens phosphorylases were subcloned, overexpressed and analyzed for their substrate specificity. DeoD(1) and PunA could use a variety of purine substrates, including an antiviral drug 2',3'-dideoxyinosine (ddI). In one-pot synthesis using Clostridium phosphorylases, 2',3'-dideoxyuridine and hypoxanthine were converted to ddI at yield of about 30%.
- Biology and Life Sciences
- ISSN: 1532-2335