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Pasteurella multocida thymidine kinase 1 efficiently activates pyrimidine nucleoside analogs.

  • Anders Ranegaard Clausen
  • S A L Al Meani
  • Jure Piskur (Professor)
Publiceringsår: 2010
Språk: Engelska
Sidor: 359-362
Publikation/Tidskrift/Serie: Nucleosides, nucleotides & nucleic acids
Volym: 29
Nummer: 4-6
Dokumenttyp: Artikel
Förlag: Taylor & Francis Group


In the Pasteurella multocida genome only one putative deoxyribonucleoside kinase encoding gene, for thymidine kinase 1 (PmTK1), was identified. The PmTK1 gene was sub-cloned into Escherichia coli KY895 and it sensitized the host towards 2',2'-difluoro-deoxycytidine (gemcitabine, dFdC), 3'-azido-thymidine (AZT) and 5-fluoro-deoxyuridine (5F-dU). PmTK1 was over-expressed and purified with two different tags. Apparently, deoxyuridine (dU), and not thymidine (dT), is the preferred substrate. We suggest that PmTK1s could be employed as a species-specific activator of uracil-based nucleoside antibiotics.



  • Biology and Life Sciences


  • ISSN: 1532-2335

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