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Proteomic analysis identifies candidate proteins associated with distant recurrences in breast cancer after adjuvant chemotherapy.

Publiceringsår: 2007
Språk: Engelska
Sidor: 1086-1093
Publikation/Tidskrift/Serie: Journal of Pharmaceutical and Biomedical Analysis
Volym: 43
Nummer: 3
Dokumenttyp: Artikel
Förlag: Elsevier B V

Sammanfattning

Breast cancer is a heterogenous disease and it is of importance to select patients with regard to different prognosis and treatment sensitivity to individualize treatment regimes. In this study we successfully adapted a protein extraction protocol from mRNA extracted tumor samples enabling two-dimensional gel electrophoresis (2-DE) analysis of samples previously analyzed by cDNA microarray. The aim was to find candidate proteins that distinguish breast cancer patients with or without recurrences after adjuvant CMF (cyclophosphamide, methotrexate and 5-FU) treatment within four years to follow-up. We identified several proteins distinguishing the recurrence group from the non-recurrence group, especially in the ER and PgR positive subgroup (n = 7). The induced proteins were involved in translation/folding, iron ion binding, and protease inhibition, whereas proteins involved in signaling, ubiquitination, and splicing were decreased in expression. These results show that it is possible to use 2-DE to separate high abundant proteins in breast cancer tissue and to find discriminating proteins to identify patients with different prognosis after adjuvant CMF treatment.

Disputation

Nyckelord

  • Medicine and Health Sciences
  • drug resistance
  • breast cancer
  • prognostic markers
  • two-dimensional gel electrophoresis

Övriga

Published
Yes
  • ISSN: 0731-7085

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