The macrophage beta-glucan receptor mediates arachidonate release induced by zymosan: Essential role for Src family kinases.
Författare
Summary, in English
Yeast-derived zymosan beads are among the classical agents used to induce sterile inflammatory responses in experimental animals and macrophage activation in cell culture. In macrophages the cytosolic phospholipase A2 becomes activated, leading to mobilization of arachidonate and the generation of prostaglandins and leukotrienes. Although zymosan can interact with several receptors it has not been unequivocally demonstrated which interaction is required for induction of the eicosanoid response. We have compared arachidonate release induced in primary mouse macrophages by zymosan and particulate beta-glucan and found striking similarities. The similarities include the effects of dectin-1 antagonists (soluble beta-glucan and laminarin) and of inhibitors of Src family kinases, the Tec kinase Btk, phosphatidylinositol 3-kinase and the Map kinases ERK and p38. Furthermore, particulate beta-glucan was equally effective as zymosan in causing phosphorylation of phospholipase C gamma 2, arguing that both agents act via the beta-glucan receptor dectin-1 and that the above signal components are engaged down-stream of that receptor. Suggestive evidence for a role of the scaffold adaptor Gab2 is also presented. (c) 2006 Elsevier Ltd. All rights reserved.
Publiceringsår
2007
Språk
Engelska
Sidor
1509-1515
Publikation/Tidskrift/Serie
Molecular Immunology
Volym
44
Issue
7
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
Pergamon Press Ltd.
Ämne
- Immunology in the medical area
Nyckelord
- Btk Gab2
- cPLA2
- dectin-1
- eicosanoids
- PLC gamma 2
Status
Published
Forskningsgrupp
- Vessel Wall Biology
ISBN/ISSN/Övrigt
- ISSN: 1872-9142