Occurrence of selenoprotein enzyme activities and mRNA in bovine mammary tissue.
Författare
Summary, in English
To elucidate the possible role of selenoproteins for milk formation and mammary gland physiology, the activities of selenoprotein enzymes and the expression of selenoprotein genes were studied in the bovine mammary gland. Messenger RNA was demonstrated for selenoprotein P, thioredoxin reductase 1, and for glutathione peroxidase (GPx) 1, 3, and 4. Significant differences in mRNA expression between the cows were seen for GPx 1 and GPx 3. The enzyme activity of glutathione peroxidase varied approximately 16-fold among cows, and the activity of thioredoxin reductase and the concentration of soluble Se varied approximately 6-fold among cows. There were positive correlations between glutathione peroxidase activity, thioredoxin reductase activity, and soluble Se, the correlation between glutathione peroxidase activity and soluble Se being the strongest. Furthermore, selenoprotein P expression correlated with GPx 1 mRNA expression and with soluble Se. There was also a correlation between glutathione peroxidase activity and the mRNA expression of GPx 1. The general conclusion from the data was that the activity of glutathione peroxidase and thioredoxin reductase and the mRNA expression of selenoprotein P and GPx 1 and 3 were influenced by Se status, but the expression of GPx 4 and thioredoxin reductase 1 were not. These results indicate that the Se status in mammary tissue is an important regulator of selenoprotein activity and expression, but that other factors are also in operation.
Publiceringsår
2007
Språk
Engelska
Sidor
918-927
Publikation/Tidskrift/Serie
Journal of Dairy Science
Volym
90
Issue
2
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
American Dairy Science Association
Ämne
- Basic Medicine
Nyckelord
- glutathione peroxidase
- selenoprotein
- mammary tissue
- thioredoxin reductase
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 1525-3198