Multisite phosphorylation of adipocyte and hepatocyte phosphodiesterase 3B.
Författare
Summary, in English
Phosphodiesterase 3B (PDE3B) is an important component of insulin and cAMP-dependent signalling pathways. In order to study phosphorylation of PDE3B, we have used an adenoviral system to express recombinant flag-tagged PDE3B in primary rat adipocytes and H4IIE hepatoma cells. Phosphorylation of PDE3B after treatment of cells with insulin, cAMP-increasing agents, or the phosphatase inhibitor, calyculin A was analyzed by two-dimensional tryptic phosphopeptide mapping and mass spectrometry. We found that PDE3B is multisite phosphorylated in adipocytes and H4IIE hepatoma cells in response to all these stimuli. Several sites were identified; serine (S)273, S296, S421, S424/5, S474 and S536 were phosphorylated in adipocyte as well as H4IIE hepatoma cells whereas S277 and S507 were phosphorylated in hepatoma cells only. Several of the sites were phosphorylated by insulin as well as cAMP-increasing hormones indicating integration of the two signalling pathways upstream of PDE3B, maybe at the level of protein kinase B.
Publiceringsår
2007
Språk
Engelska
Sidor
584-592
Publikation/Tidskrift/Serie
Biochimica et Biophysica Acta: Molecular Cell Research
Volym
1773
Issue
4
Fulltext
- Available as PDF - 892 kB
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Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
Elsevier
Ämne
- Endocrinology and Diabetes
Nyckelord
- Phosphorylation
- PDE3B
- Adipocyte
- Insulin
- Hepatocyte
- cAMP
Status
Published
Forskningsgrupp
- Insulin Signal Transduction
ISBN/ISSN/Övrigt
- ISSN: 0167-4889