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The Transcriptional Activity of Neurog3 Affects Migration and Differentiation of Ectopic Endocrine Cells in Chicken Endoderm

Författare:
  • Louise C. Rosenberg
  • Merete L. Lafon
  • Jesper Pedersen
  • Hani Yassin
  • Jan Nygaard Jensen
  • Palle Serup
  • Jacob Hecksher-Sorensen
Publiceringsår: 2010
Språk: Engelska
Sidor: 1950-1966
Publikation/Tidskrift/Serie: Developmental Dynamics
Volym: 239
Nummer: 7
Dokumenttyp: Artikel
Förlag: Wiley

Sammanfattning

Neurog3 is expressed transiently in pancreatic endocrine progenitors where it is responsible for activating a transcription factor cascade which eventually defines the mature endocrine cells. However, the mechanism by which Neurog3 regulates different aspects of the endocrine differentiation program is less clear. In this report we used in ovo electroporation to investigate how manipulation of Neurog3 protein activity affected migration, differentiation and fate determination. We found that changes in the onset of Neurog3 expression only had minor effect on differentiation. However increasing the transcriptional activity of Neurog3 by fusing it to VP16 or co-electroporating with Ep300 caused the electroporated cells to migrate rather than differentiate. In contrast, reducing the transcriptional activity of Neurog3 by deleting parts of the activation domain, by fusing Neurog3 to the engrailed repressor domain, or co-electroporating with Hdac1 greatly increased the proportion of glucagon expressing cells. Developmental Dynamics 239:1950-1966, 2010. (C) 2010 Wiley-Liss, Inc.

Disputation

Nyckelord

  • Biology and Life Sciences
  • endocrine competence
  • glucagon
  • differentiation
  • pancreas
  • fluorescence
  • whole-mount
  • in ovo electroporation
  • neurogenin 3
  • Neurog3
  • Ngn3

Övrigt

Published
Yes
  • ISSN: 1058-8388

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