In-Source Decay Causes Artifacts in SELDI-TOF MS Spectra.
Författare
Summary, in English
SELDI-TOF MS is a mass spectrometric technique which has been extensively used for biomarker discovery. In this study, we show that in-source decay is an important source for the generation of additional spectral peaks with this technique, both for pure proteins and proteins in serum samples. Thus, SELDI-TOF MS could be used to gain sequence information from proteins, but the results also question the uncritical use of SELDI-TOF MS as a general method for the detection of biomarkers.
Avdelning/ar
Publiceringsår
2007
Språk
Engelska
Sidor
1609-1614
Publikation/Tidskrift/Serie
Journal of Proteome Research
Volym
6
Issue
4
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
The American Chemical Society (ACS)
Ämne
- Cancer and Oncology
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 1535-3893