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Immunohistochemical Distribution of Cyclic GMP-Dependent Protein Kinase-1 in Human Prostate Tissue.

In English

Författare

Summary, in English

Objectives: Phosphodiesterase 5 (PDE5) inhibitors improve smooth muscle relaxation and therefore are considered for pharmacotherapy of benign prostatic hyperplasia (BPH) and lower urinary tract symptoms (LUTS). Cyclic guanosine monophosphate (cGMP) -dependent protein kinase-1 (cGKI) has been identified as one of the downstream targets for cGMP. The aim of the present study was to evaluate, by means of immunohistochemistry and Western blot analysis, the expression and localization of cGKI isoforms in relation to smooth muscle alpha-actin and cGMP in the human prostate. Methods: Cryostat sections of tissue segments excised from the transition zone of human prostates from 11 patients (aged 54-68 yr) were incubated with primary antibodies directed against smooth muscle alpha-actin, cGMP, cGKI, c:GKI alpha, and cGKI beta. Visualization of double-labelled immunofluorescent staining was achieved by laser microscopy. Western blot analysis was performed to confirm the expression of cGKI isoforms. Results: Immunoreactivities specific for cGKI, cGKI alpha, and cGKI beta were observed in the smooth musculature of the transition zone. Double-staining revealed the colocalization of smooth muscle alpha-actin, cGMP, and cGKI isoforms in smooth muscle cells of the fibromuscular stroma. The expression of cGKI isoforms was confirmed by Western blot analysis. Conclusions: Our results confirm the presence of cGKI isoforms a and P in the transition zone of human prostate tissue. in addition, the colocalization of alpha-actin, cGMP, and cGKI isoforms provides further evidence for a significant role of the nitric oxide/cGMP pathway in the regulation of smooth muscle contractility in human prostate tissue and therefore could provide additional targets for pharmacotherapy of BPH and LUTS. (C) 2007 European Association of Urology. Published by Elsevier B.V. All rights reserved.

Publiceringsår

2007

Språk

Engelska

Sidor

495-502

Publikation/Tidskrift/Serie

European Urology

Volym

52

Issue

2

Dokumenttyp

Artikel i tidskrift

Förlag

Elsevier

Ämne

  • Urology and Nephrology

Nyckelord

  • cyclic nucleotides
  • benign prostatic hyperplasia
  • lower urinary tract
  • symptoms
  • phosphodiesterase 5
  • phosphodiesterase 5 inhibitor
  • protein
  • kinase G

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1873-7560