The decorin sequence SYIRIADTNIT binds collagen type I.

Summary, in English

Decorin belongs to the small leucine-rich repeat proteoglycan family, interacts with fibrillar collagens, and regulates the assembly, structure, and biomechanical properties of connective tissues. The decorin-collagen type I-binding region is located in leucine-rich repeats 5–6. Site-directed mutagenesis of this 54-residue-long collagen-binding sequence identifies Arg-207 and Asp-210 in leucine-rich repeat 6 as crucial for the binding to collagen. The synthetic peptide SYIRIADTNIT, which includes Arg-207 and Asp-210, inhibits the binding of full-length recombinant decorin to collagen in vitro. These collagen-binding amino acids are exposed on the exterior of the beta-sheet-loop structure of the leucine-rich repeat. This resembles the location of interacting residues in other leucine-rich repeat proteins.

• Författare: Sebastian Kalamajski; Anders Aspberg; Åke Oldberg
• Avdelning/ar: Åke Oldberg´s group; Institutionen för experimentell medicinsk vetenskap
• Publiceringsår: 2007
• Språk: Engelska
• Sidor: 16062-16067
• Publikation/Tidskrift/Serie: Journal of Biological Chemistry
• Volym: 282
• Issue: 22
• Länkar: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17426031&dopt=Abstract; http://dx.doi.org/10.1074/jbc.M700073200
• Dokumenttyp: Artikel i tidskrift
• Förlag: American Society for Biochemistry and Molecular Biology
• Ämne: Basic Medicine
• Status: Published
• Forskningsgrupp: Åke Oldberg´s group
• ISBN/ISSN/Övrigt: ISSN: 1083-351X