Differences in islet-enriched miRNAs in healthy and glucose intolerant human subjects.

Summary, in English

Many microRNAs (miRNAs) are known to be cell-type specific and are implicated in development of diseases. We investigated the global expression pattern of miRNAs in human pancreatic islets compared to liver and skeletal muscle, using bead-based technology and quantitative RT-PCR. In addition to the known islet-specific miR-375, we also found enrichment of miR-127-3p, miR-184, miR-195 and miR-493∗ in the pancreatic islets. The expression of miR-375, miR-127-3p, miR-184 and the liver-enriched miR-122 were positively correlated to insulin biosynthesis, while the expression of miR-127-3p and miR-184 were negatively correlated to glucose-stimulated insulin secretion (GSIS). These correlations were absent in islets of glucose intolerant donors (HbA1c⩾6.1). We suggest the presence of an islet-specific miRNA network, which consists of at least miR-375, miR-127-3p and miR-184, potentially involved in insulin secretion. Our results provide new insight into miRNA-mediated regulation of insulin secretion in healthy and glucose intolerant subjects.

• Författare: Caroline Bolmeson; Jonathan Esguerra; S Albert Salehi; Dina Speidel; Lena Eliasson; Corrado Cilio
• Avdelning/ar: Create Health; Evolutionär ekologi; Diabetes - öcellsexocytos; Islet cell physiology; Diabetes - immunovirologi; EXODIAB: Excellence of Diabetes Research in Sweden
• Publiceringsår: 2011
• Språk: Engelska
• Sidor: 16-22
• Publikation/Tidskrift/Serie: Biochemical and Biophysical Research Communications
• Volym: Dec
• Länkar: http://www.ncbi.nlm.nih.gov/pubmed/21094635?dopt=Abstract; http://dx.doi.org/10.1016/j.bbrc.2010.11.024
• Dokumenttyp: Artikel i tidskrift
• Förlag: Elsevier
• Ämne: Biological Sciences
• Status: Published
• Forskningsgrupp: Diabetes - Islet Cell Exocytosis; Islet cell physiology; Diabetes - Immunovirology
• ISBN/ISSN/Övrigt: ISSN: 1090-2104