Mechanism of NADH transfer among dehydrogenases
Publikation/Tidskrift/Serie: Biochim Biophys Acta
Steady-state and transient-state kinetic experiments have been performed to test the proposal that there is a direct (channelled) transfer of NADH from one dehydrogenase to another if the two enzymes exhibit distinct chiral coenzyme specificity (A-side vs. B-side). The results lend no support to this proposal, but are fully consistent with a free-diffusion mechanism of NADH transfer irrespective of the chiral specificity of the enzymes.
- Medicine and Health Sciences
- Alcohol Dehydrogenase/metabolism Animals Buffers Cattle Glycerolphosphate Dehydrogenase/metabolism Kinetics NAD/metabolism* Oxidoreductases/metabolism* Rabbits Stereoisomerism Swine