Size separations of starch of different botanical origin studied by asymmetrical-flow field-flow fractionation and multiangle light scattering.

Summary, in English

Asymmetrical-flow field-flow fractionation combined with multiangle light scattering and refractive index detection has been revealed to be a powerful tool for starch characterization. It is based on size separation according to the hydrodynamic diameter of the starch components. Starch from a wide range of different botanical sources were studied, including normal starch and high-amylose and high-amylopectin starch. The starch was dissolved by heat treatment at elevated pressure in a laboratory autoclave. This gave clear solutions with no granular residues. Amylose retrogradation was prevented by using freshly dissolved samples. Programmed cross flow starting at 1.0 mL min(-1) and decreasing exponentially with a half-life of 4 min was utilised. The starches showed two size populations representing mainly amylose and mainly amylopectin with an overlapping region where amylose and amylopectin were possibly co-eluted. Most of the first population had molar masses below 10(6) g mol(-1), and most of the second size population had molar masses above 10(7) g mol(-1). Large differences were found in the relative amounts of the two populations, the molar mass, and hydrodynamic diameters, depending on the plant source and its varieties.

• Författare: Karl-Gustav Wahlund; Mats Leeman; Stalin Santacruz
• Avdelning/ar: Centrum för analys och syntes; Kemiska institutionen
• Publiceringsår: 2011
• Språk: Engelska
• Sidor: 1455-1465
• Publikation/Tidskrift/Serie: Analytical and Bioanalytical Chemistry
• Volym: 399
• Länkar: http://dx.doi.org/10.1007/s00216-010-4438-5
• Dokumenttyp: Artikel i tidskrift
• Förlag: Springer
• Ämne: Analytical Chemistry
• Status: Published
• ISBN/ISSN/Övrigt: ISSN: 1618-2642