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TNF-alpha suppresses the PDGF beta-receptor kinase

Författare

Summary, in English

PDGF and TNF-alpha are both known to play important roles in inflammation, albeit frequently by opposing actions. Typically, TNF-alpha can attenuate PDGF beta-receptor signaling. Pretreatment of mouse 3T3 L1 fibroblasts with TNF-alpha greatly diminished their proliferative response to PDGF. However, TNF-alpha affected neither the binding of PDGF-BB to cell surface receptors nor the total amount of PDGF beta-receptor in the cells, but decreased the PDGF-induced in vitro kinase activity of the receptor. The phosphatase inhibitor ortho-vanadate did not prevent this effect. Ortho-phosphate labeling of cells prior to TNF-alpha treatment and PDGF-BB stimulation confirmed a decrease of in vivo phosphorylation of the PDGF beta-receptor. Two-dimensional mapping after tryptic cleavage as well as phosphoamino acid analysis demonstrated a general decrease in phosphorylation of all known tyrosine residues in the PDGF beta-receptor. The exact mechanism for this suppression remains to be clarified

Publiceringsår

2000

Språk

Engelska

Sidor

65-71

Publikation/Tidskrift/Serie

Experimental Cell Research

Volym

258

Issue

1

Dokumenttyp

Artikel i tidskrift

Förlag

Academic Press

Ämne

  • Medicinal Chemistry

Nyckelord

  • 3T3 Cells Animals Cell Division/drug effects Kinetics Mice Platelet-Derived Growth Factor/pharmacokinetics/*pharmacology Receptor Protein-Tyrosine Kinases/*antagonists & inhibitors Receptor
  • Platelet-Derived Growth Factor beta/drug effects/*physiology Signal Transduction/drug effects Transforming Growth Factor beta/*pharmacology Vanadates/pharmacology

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1090-2422