TNF-alpha suppresses the PDGF beta-receptor kinase
Författare
Summary, in English
PDGF and TNF-alpha are both known to play important roles in inflammation, albeit frequently by opposing actions. Typically, TNF-alpha can attenuate PDGF beta-receptor signaling. Pretreatment of mouse 3T3 L1 fibroblasts with TNF-alpha greatly diminished their proliferative response to PDGF. However, TNF-alpha affected neither the binding of PDGF-BB to cell surface receptors nor the total amount of PDGF beta-receptor in the cells, but decreased the PDGF-induced in vitro kinase activity of the receptor. The phosphatase inhibitor ortho-vanadate did not prevent this effect. Ortho-phosphate labeling of cells prior to TNF-alpha treatment and PDGF-BB stimulation confirmed a decrease of in vivo phosphorylation of the PDGF beta-receptor. Two-dimensional mapping after tryptic cleavage as well as phosphoamino acid analysis demonstrated a general decrease in phosphorylation of all known tyrosine residues in the PDGF beta-receptor. The exact mechanism for this suppression remains to be clarified
Publiceringsår
2000
Språk
Engelska
Sidor
65-71
Publikation/Tidskrift/Serie
Experimental Cell Research
Volym
258
Issue
1
Dokumenttyp
Artikel i tidskrift
Förlag
Academic Press
Ämne
- Medicinal Chemistry
Nyckelord
- 3T3 Cells Animals Cell Division/drug effects Kinetics Mice Platelet-Derived Growth Factor/pharmacokinetics/*pharmacology Receptor Protein-Tyrosine Kinases/*antagonists & inhibitors Receptor
- Platelet-Derived Growth Factor beta/drug effects/*physiology Signal Transduction/drug effects Transforming Growth Factor beta/*pharmacology Vanadates/pharmacology
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 1090-2422