Identification of Tyr-703 and Tyr-936 as the primary association sites for Grb2 and Grb7 in the c-Kit/stem cell factor receptor
Författare
Summary, in English
In this paper we demonstrate the presence of two novel in vivo autophosphorylation sites in the c-Kit/stem cell factor receptor (c-Kit/SCFR): Tyr-703 in the kinase insert and Tyr-936 in the C-terminal tail. We furthermore demonstrate that the adapter protein Grb2 is a specific binding partner for both phosphorylated Tyr-703 and phosphorylated Tyr-936, whereas the adapter protein Grb7 binds selectively to phosphorylated Tyr-936. It is shown that the association occurs through the Src homology 2 (SH2) domains of Grb2 and Grb7. Binding of Grb2 to Tyr-703 in the c-Kit/SCFR provides a link to the Ras/mitogen-activated protein kinase pathway.
Publiceringsår
1999
Språk
Engelska
Sidor
211-216
Publikation/Tidskrift/Serie
Biochemical Journal
Volym
341
Issue
1
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
Portland Press
Ämne
- Medicinal Chemistry
Nyckelord
- Signal Transducing Cells
- *Adaptor Proteins
- Cultured Endothelium
- Vascular/cytology GRB2 Adaptor Protein GRB7 Adaptor Protein Humans Phosphorylation Protein Binding Proteins/*metabolism Proto-Oncogene Proteins c-kit/genetics/*metabolism Receptor Protein-Tyrosine Kinases/genetics/metabolism Recombinant Fusion Proteins/metabolism Stem Cell Factor *Tyrosine src Homology Domains
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 0264-6021