Identification of two C-terminal autophosphorylation sites in the PDGF beta-receptor: involvement in the interaction with phospholipase C-gamma
Författare
Summary, in English
Two novel sites of autophosphorylation were localized to the C-terminal tail of the PDGF beta-receptor. To evaluate the importance of these phosphorylation sites, receptor mutants in which Tyr1009, Tyr1021 or both were replaced with phenylalanine residues, were expressed in porcine aortic endothelial (PAE) cells. These mutants were similar to the wild type receptor with regard to protein tyrosine kinase activity and ability to induce mitogenicity in response to PDGF-BB. However, both the Y1009F and Y1021F mutants showed a decreased ability to mediate association with and the tyrosine phosphorylation of phospholipase C-gamma (PLC-gamma) compared to the wild type PDGF beta-receptor; in the case of the Y1009F/Y1021F double mutant, no association or phosphorylation of PLC-gamma could be detected. These data show that tyrosine phosphorylation of PLC-gamma is dependent on autophosphorylation of the PDGF beta-receptor at Tyr1009 and Tyr1021.
Publiceringsår
1992
Språk
Engelska
Sidor
3911-3919
Publikation/Tidskrift/Serie
EMBO Journal
Volym
11
Issue
11
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
Oxford University Press
Ämne
- Medicinal Chemistry
Nyckelord
- Amino Acid Sequence Animals Base Sequence Cell Line Female Isoenzymes/*metabolism Kinetics Macromolecular Substances Models
- Site-Directed Oligodeoxyribonucleotides Peptide Fragments/isolation & purification Peptide Mapping Phosphates/*metabolism Phosphopeptides/isolation & purification Phosphorylation Plasmids Platelet-Derived Growth Factor/*metabolism Receptors
- Structural Molecular Sequence Data Mutagenesis
- Platelet-Derived Growth Factor/genetics/*metabolism Recombinant Proteins/metabolism Swine Transfection Type C Phospholipases/*metabolism Uterus/metabolism
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 1460-2075