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Ten polymorphic microsatellite markers for Hieracium s.s. (Asteraceae)

Publiceringsår: 2010
Språk: Engelska
Sidor: 295-300
Publikation/Tidskrift/Serie: Conservation Genetics Resources
Volym: 2
Nummer: 1
Dokumenttyp: Artikel
Förlag: Springer

Sammanfattning

Eight novel microsatellite markers were developed for Hieracium s.s. (excluding Pilosella) using a modified FIASCO protocol, and two out of nine microsatellites that were originally developed for Pilosella officinarum were optimised for Hieracium s.s.. All ten microsatellites consistently amplified loci from a wide range of representatives from north and central European species of various sections of the genus. When tested on 19 individuals, nine microsatellites were found to be highly polymorphic with 6–29 alleles, while one microsatellite marker was monomorphic. Cross-amplification showed that six of the ten microsatellites also amplified products in one taxon of Pilosella, P. floribunda × officinarum.

Disputation

Nyckelord

  • Biology and Life Sciences
  • microsatellite marker
  • FIASCO
  • Hieracium
  • Pilosella
  • genetic variation
  • cross-amplification

Övriga

Published
Yes
  • Molecular Ecology and Evolution Lab
  • ISSN: 1877-7252

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