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Expression of MMP-10 in lung cancer

In English

Författare

  • Xiaoying Zhang
  • Shaowei Zhu
  • Guanghua Luo
  • Lu Zheng
  • Jiang Wei
  • Jiang Zhu
  • Qingfeng Mu
  • Ning Xu

Summary, in English

Background: Matrix metalloproteinase (MMP)-mediated degradation of the extracellular matrix is a key point in tumor development and expansion. MMP-10 is one of the most important and well-characterized members of the MMP family. In the present study, we examined MMP-10 mRNA and protein levels in non-small cell lung cancer (NSCLC). Patients and Methods: Three endogenous reference genes including GAPDH, beta-actin and 18S rRNA, and MMP-10 mRNA levels were determined using real-time RT-PCR. Immunohistochemical staining was applied to examine MMP-10 protein levels. Both tumor and adjacent normal lung tissues were collected from 32 NSCLC patients. The mRNA levels of GAPDH, beta-actin and 18S rRNA exhibited great differences in tumor tissues and in the adjacent normal tissues. The ratio of mRNA levels in the tumor tissues compared to the adjacent normal tissues followed the pattern GAPDH > beta-actin > 18S rRNA. Thereafter, we chose 18S rRNA as the reference gene for MMP-10 mRNA level determinations. MMP-10 mRNA levels in tumor tissues were significantly lower than those in the adjacent normal tissues (p = 0.0423). However, the MMP- 10 protein levels were higher in the tumor tissues than in the adjacent normal tissues (p=0.0055). The MMP-10 mRNA level was positively-correlated to the MMP-10 protein level in tumor tissues (r=0.4672, p=0.0161), but this correlation was not seen in the adjacent normal tissues (r=-0.0030, p=0.9891). Conclusion: There were no statistical differences in MMP-10 mRNA levels and protein levels in relation to patient's gender, age, tumor stages, tumor size, lymph node metastasis or tumor histological type.

Publiceringsår

2007

Språk

Engelska

Sidor

2791-2795

Publikation/Tidskrift/Serie

Anticancer research

Volym

27

Issue

4C

Dokumenttyp

Artikel i tidskrift

Förlag

International Institute of Cancer Research

Ämne

  • Cancer and Oncology

Nyckelord

  • real-time RT-PCR
  • endogenous reference gene
  • MMP-10
  • NSCLC
  • immunohistochemistry

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1791-7530