Complimentary action : C1q increases ganglion cell survival in an in vitro model of retinal degeneration
Författare
Summary, in English
Using a previously described retinal explant culture system as an acute injury model, we here explore the role of C1q, the initiator of the classical complement pathway, in neuronal cell survival and retinal homeostasis. Full-thickness adult rat retinal explants were divided into four groups, receiving the following supplementation: C1q (50 nM), C1-inhibitor (C1-inh; Berinert; 500 mg/l), C1q + C1-inh, and no supplementation (culture controls). Explants were kept for 12 h or 2 days after which they were examined morphologically and with a panel of immunohistochemical markers. C1q supplementation protects ganglion cells from degeneration within the explant in vitro system. This effect is correlated to an attenuated endogenous production of C1q, and a quiesced gliotic response.
Avdelning/ar
Publiceringsår
2016-09-15
Språk
Engelska
Sidor
117-129
Publikation/Tidskrift/Serie
Journal of Neuroimmunology
Volym
298
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
Elsevier
Ämne
- Cell and Molecular Biology
- Ophthalmology
Nyckelord
- Adult retina
- C1q
- Ganglion cell
- Neuroinflammation
- Retinal degeneration
- Retinal explant culture
Status
Published
Forskningsgrupp
- Protein Chemistry, Malmö
ISBN/ISSN/Övrigt
- ISSN: 0165-5728