Webbläsaren som du använder stöds inte av denna webbplats. Alla versioner av Internet Explorer stöds inte längre, av oss eller Microsoft (läs mer här: * https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Var god och använd en modern webbläsare för att ta del av denna webbplats, som t.ex. nyaste versioner av Edge, Chrome, Firefox eller Safari osv.

Cytological and molecular description of Hamiltosporidium tvaerminnensis gen. et sp nov., a microsporidian parasite of Daphnia magna, and establishment of Hamiltosporidium magnivora comb. nov.

In English

Författare

Summary, in English

We describe the new microsporidium Hamiltosporidium tvaerminnensis gen. et sp. nov. with an emphasis on its ultrastructural characteristics and phylogenetic position as inferred from the sequence data of SSU rDNA, alpha-and beta-tubulin. This parasite was previously identified as Octosporea bayeri Jirovec, 1936 and has become a model system to study the ecology, epidemiology, evolution and genomics of microsporidia - host interactions. Here, we present evidence that shows its differences from O. bayeri. Hamiltosporidium tvaerminnensis exclusively infects the adipose tissue, the ovaries and the hypodermis of Daphnia magna and is found only in host populations located in coastal rock pool populations in Finland and Sweden. Merogonial stages of H. tvaerminnensis have isolated nuclei; merozoites are formed by binary fission or by the cleaving of a plasmodium with a small number of nuclei. A sporogonial plasmodium with isolated nuclei yields 8 sporoblasts. Elongated spores are generated by the most finger-like plasmodia. The mature spores are polymorphic in shape and size. Most spores are pyriform (4.9-5.6x2.2-2.3 mu m) and have their polar filament arranged in 12-13 coils. A second, elongated spore type (6.8-12.0x1.6-2.1 mu m) is rod-shaped with blunt ends and measures 6.8-12.0x1.6-2.1 mu m. The envelope of the sporophorous vesicle is thin and fragile, formed at the beginning of the sporogony. Cytological and molecular comparisons with Flabelliforma magnivora, a parasite infecting the same tissues in the same host species, reveal that these two species are very closely related, yet distinct. Moreover, both cytological and molecular data indicate that these species are quite distant from F. montana, the type species of the genus Flabelliforma. We therefore propose that F. magnivora also be placed in Hamiltosporidium gen. nov.

Publiceringsår

2011

Språk

Engelska

Sidor

447-462

Publikation/Tidskrift/Serie

Parasitology

Volym

138

Issue

4

Dokumenttyp

Artikel i tidskrift

Förlag

Cambridge University Press

Ämne

  • Zoology

Nyckelord

  • Ultrastructural characteristics
  • molecular phylogeny
  • Octosporea
  • bayeri
  • Flabelliforma magnivora
  • Microsporidia
  • Cladocerca

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1469-8161