Visualisation of ectomycorrhizal rhizomorph structure using laser scanning confocal microscopy

Summary, in English

A method for the observation of the three-dimensional structure of intact ectomycorrhizal rhizomorphs is described. The method is based on a combination of clearing the material with KOH followed by staining with congo red and subsequent imaging under a laser scanning confocal microscope (LSCM). The images obtained are of a much higher three-dimensional resolution than those obtained previously by use of conventional light microscopical techniques. The structure of highly differentiated and undifferentiated rhizomorphs is described. Applications of the method are briefly discussed.

• Författare: P F Schweiger; Hervé Rouhier; Bengt Söderström
• Avdelning/ar: MEMEG; Mikrobiologisk ekologi
• Publiceringsår: 2002
• Språk: Engelska
• Sidor: 349-354
• Publikation/Tidskrift/Serie: Mycological Research
• Volym: 106
• Issue: 3
• Fulltext: - 334 kB; Download statistics
• Länkar: http://dx.doi.org/10.1017/S0953756202005579
• Dokumenttyp: Artikel i tidskrift
• Förlag: Cambridge University Press
• Ämne: Biological Sciences
• Status: Published
• Forskningsgrupp: Microbial Ecology
• ISBN/ISSN/Övrigt: ISSN: 0953-7562