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Visualisation of ectomycorrhizal rhizomorph structure using laser scanning confocal microscopy

Publiceringsår: 2002
Språk: Engelska
Sidor: 349-354
Publikation/Tidskrift/Serie: Mycological Research
Volym: 106
Nummer: 3
Dokumenttyp: Artikel i tidskrift
Förlag: Cambridge University Press


A method for the observation of the three-dimensional structure of intact ectomycorrhizal rhizomorphs is described. The method is based on a combination of clearing the material with KOH followed by staining with congo red and subsequent imaging under a laser scanning confocal microscope (LSCM). The images obtained are of a much higher three-dimensional resolution than those obtained previously by use of conventional light microscopical techniques. The structure of highly differentiated and undifferentiated rhizomorphs is described. Applications of the method are briefly discussed.


  • Biological Sciences


  • Microbial Ecology
  • ISSN: 1469-8102

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