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Essential role of DivIVA in polar growth and morphogenesis in Streptomyces coelicolor A3(2)

Författare:
Publiceringsår: 2003
Språk: Engelska
Sidor: 1523-1536
Publikation/Tidskrift/Serie: Molecular Microbiology
Volym: 49
Nummer: 6
Dokumenttyp: Artikel i tidskrift
Förlag: Wiley-Blackwell

Sammanfattning

Streptomycetes grow by cell wall extension at hyphal

tips. The molecular basis for such polar growth in

prokaryotes is largely unknown. It is reported here

that DivIVA

SC

, the

Streptomyces coelicolor

homologue

of the

Bacillus subtilis

protein DivIVA, is essential

and directly involved in hyphal tip growth and

morphogenesis. A DivIVA

SC

-EGFP hybrid was distinctively

localized to hyphal tips and lateral branches.

Reduction of

divIVA

SC

expression to about 10% of the

normal level produced a phenotype strikingly similar

to that of many tip growth mutants in fungi, including

irregular curly hyphae and apical branching. Overexpression

of the gene dramatically perturbed determination

of cell shape at the growing tips. Furthermore,

staining of nascent peptidoglycan with a fluorescent

vancomycin conjugate revealed that induction of

overexpression in normal hyphae disturbed tip

growth, and gave rise to several new sites of cell wall

assembly, effectively causing hyperbranching. The

results show that DivIVA

SC

is a novel bacterial morphogene,

and it is localized at or very close to the

apical sites of peptidoglycan assembly in

Streptomyces

hyphae.

Nyckelord

  • Biological Sciences

Övriga

Published
  • ISSN: 1365-2958

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