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Exploiting sequence space : Shuffling in vivo formed complementarity determining regions into a master framework

In English

Författare

Summary, in English

A novel approach in molecular design is presented, where in vivo formed complementarity determining regions (CDR) from antibody genes were shuffled into a specific framework region. A synthetic gene library of soluble VH-fragments was created and the complexity of the library was determined by sequencing. The synthetic genes were diverse and contained random combinations of CDR from different germlines. All CDR were randomised in one step and by using in vivo formed CDR, the length, sequence and combination were varied simultaneously.

Publiceringsår

1998-07-30

Språk

Engelska

Sidor

471-476

Publikation/Tidskrift/Serie

Gene

Volym

215

Issue

2

Dokumenttyp

Artikel i tidskrift

Förlag

Elsevier

Nyckelord

  • CDR shuffling
  • Gene assembly
  • Immunoglobulin germline gene
  • Primer design
  • Synthetic gene library

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 0378-1119