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MRM assay for quantitation of complement components in human blood plasma - a feasibility study on multiple sclerosis.

Publiceringsår: 2011
Språk: Engelska
Sidor: 211-220
Publikation/Tidskrift/Serie: Journal of Proteomics
Volym: 75
Nummer: 1
Dokumenttyp: Artikel
Förlag: Elsevier

Sammanfattning

As a proof-of-principle study, a multiple reaction monitoring (MRM) assay was developed for quantitation of proteotypic peptides, representing seven plasma proteins associated with inflammation (complement components and C-reactive protein). The assay development and the sample analysis were performed on a linear ion trap mass spectrometer. We were able to quantify 5 of the 7 target proteins in depleted plasma digests with reasonable reproducibility over a 2 orders of magnitude linear range (RSD < 25%). The assay panel was utilized for the analysis of a small multiple sclerosis sample cohort with 10 diseased and 8 control patients.

Disputation

Nyckelord

  • Medicine and Health Sciences
  • linear ion trap
  • multiple sclerosis
  • selective reaction monitoring

Övriga

Published
Yes
  • ISSN: 1876-7737

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