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Proteinases of common pathogenic bacteria degrade and inactivate the antibacterial peptide LL-37

In English

Författare

Summary, in English

Effectors of the innate immune system, the anti-bacterial peptides, have pivotal roles in preventing infection at epithelial surfaces. Here we show that proteinases of the significant human pathogens Pseudomonas aeruginosa, Enterococcus faecalis, Proteus mirabilis and Streptococcus pyogenes, degrade the antibacterial peptide LL-37. Analysis by mass spectrometry of fragments generated by P. aeruginosa elastase in vitro revealed that the initial cleavages occurred at Asn-Leu and Asp-Phe, followed by two breaks at Arg-Ile, thus inactivating the peptide. Proteinases of the other pathogens also degraded LL-37 as determined by SDS-PAGE. Ex vivo, P. aeruginosa elastase induced LL-37 degradation in human wound fluid, leading to enhanced bacterial survival. The degradation was blocked by the metalloproteinase inhibitors GM6001 and 1, 10-phenantroline (both of which inhibited P. aeruginosa elastase, P. mirabilis proteinase, and E. faecalis gelatinase), or the inhibitor E64 (which inhibited S. pyogenes cysteine proteinase). Additional experiments demonstrated that dermatan sulphate and disaccharides of the structure [DeltaUA(2S)-GalNAc(4,6S)], or sucroseoctasulphate, in-hibited the degradation of LL-37. The results indicate that proteolytic degradation of LL-37 is a common virulence mechanism and that molecules which block this degradation could have therapeutic potential.

Publiceringsår

2002

Språk

Engelska

Sidor

157-168

Publikation/Tidskrift/Serie

Molecular Microbiology

Volym

46

Issue

1

Dokumenttyp

Artikel i tidskrift

Förlag

Wiley-Blackwell

Ämne

  • Dermatology and Venereal Diseases

Nyckelord

  • Endopeptidases/*metabolism
  • Glycosaminoglycans/pharmacology
  • Microbial Sensitivity Tests
  • Human
  • Molecular Sequence Data
  • Pancreatic Elastase/metabolism
  • Neutrophils
  • Protease Inhibitors/pharmacology
  • Pseudomonas aeruginosa/enzymology/pathogenicity
  • Spectrum Analysis
  • Mass
  • Sulfur/metabolism
  • Wound Infection/microbiology
  • Virulence
  • Disaccharides/chemistry/pharmacology
  • Bacterial Infections/microbiology
  • Bacteria/*drug effects/*metabolism/pathogenicity
  • Antimicrobial Cationic Peptides/genetics/*metabolism/*pharmacology
  • Amino Acid Sequence

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1365-2958