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Use and misuse of PLFA measurements in soils

Publiceringsår: 2011
Språk: Engelska
Sidor: 1621-1625
Publikation/Tidskrift/Serie: Soil Biology & Biochemistry
Volym: 43
Nummer: 8
Dokumenttyp: Artikel
Förlag: Elsevier Science Ltd


The determination of the phospholipid fatty acid (PLFA) pattern of soil organisms has become one of the most commonly used methods to study microbial community structure. Here we recapitulate the background of our work applying the PLFA method to soil in the early 1990s. We also stress that although the PLFA method was, and still is, a rapid and sensitive method to detect changes in the microbial community in soil, as with all popular methods it can be misused. We discuss problems in PLFA interpretation, the extent of turn-over of PLFAs in soil, and the flawed use of diversity indices to evaluate PLFA patterns. (C) 2010 Elsevier Ltd. All rights reserved.



  • Biology and Life Sciences
  • Soil
  • Community structure
  • PLFA
  • NLFA
  • Heavy metals
  • pH


  • BECC
  • Microbial Ecology
  • ISSN: 0038-0717

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