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Dekkera/Brettanomyces yeasts for ethanol production from renewable sources under oxygen-limited and low-pH conditions

Författare:
  • Silvia Galafassi
  • Annamaria Merico
  • Francesca Pizza
  • Linda Hellborg
  • Francesco Molinari
  • Jure Piskur (Professor)
  • Concetta Compagno
Publiceringsår: 2011
Språk: Engelska
Sidor: 1079-1088
Publikation/Tidskrift/Serie: Journal of Industrial Microbiology & Biotechnology
Volym: 38
Nummer: 8
Dokumenttyp: Artikel
Förlag: Springer

Sammanfattning

Industrial fermentation of lignocellulosic hydrolysates to ethanol requires microorganisms able to utilise a broad range of carbon sources and generate ethanol at high yield and productivity. D. bruxellensis has recently been reported to contaminate commercial ethanol processes, where it competes with Saccharomyces cerevisiae [4, 26]. In this work Brettanomyces/Dekkera yeasts were studied to explore their potential to produce ethanol from renewable sources under conditions suitable for industrial processes, such as oxygen-limited and low-pH conditions. Over 50 strains were analysed for their ability to utilise a variety of carbon sources, and some strains grew on cellobiose and pentoses. Two strains of D. bruxellensis were able to produce ethanol at high yield (0.44 g g(-1) glucose), comparable to those reported for S. cerevisiae. B. naardenensis was shown to be able to produce ethanol from xylose. To obtain ethanol from synthetic lignocellulosic hydrolysates we developed a two-step fermentation strategy: the first step under aerobic conditions for fast production of biomass from mixtures of hexoses and pentoses, followed by a second step under oxygen limitation to promote ethanol production. Under these conditions we obtained biomass and ethanol production on synthetic lignocellulosic hydrolysates, with ethanol yields ranging from 0.2 to 0.3 g g(-1) sugar. Hexoses, xylose and arabinose were consumed at the end of the process, resulting in 13 g l(-1) of ethanol, even in the presence of furfural. Our studies showed that Brettanomyces/Dekkera yeasts have clear potential for further development for industrial processes aimed at production of ethanol from renewable sources.

Disputation

Nyckelord

  • Biology and Life Sciences
  • Ethanol
  • Dekkera
  • Brettanomyces
  • Pentoses
  • Renewable sources

Övriga

Published
Yes
  • ISSN: 1367-5435

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