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Visualisation of ectomycorrhizal rhizomorph structure using laser scanning confocal microscopy

Författare:
Publiceringsår: 2002
Språk: Engelska
Sidor: 349-354
Publikation/Tidskrift/Serie: Mycological Research
Volym: 106
Nummer: 3
Dokumenttyp: Artikel
Förlag: Elsevier B.V.

Sammanfattning

A method for the observation of the three-dimensional structure of intact ectomycorrhizal rhizomorphs is described. The method is based on a combination of clearing the material with KOH followed by staining with congo red and subsequent imaging under a laser scanning confocal microscope (LSCM). The images obtained are of a much higher three-dimensional resolution than those obtained previously by use of conventional light microscopical techniques. The structure of highly differentiated and undifferentiated rhizomorphs is described. Applications of the method are briefly discussed.

Disputation

Nyckelord

  • Biology and Life Sciences

Övriga

Published
Yes
  • Microbial Ecology
  • ISSN: 0953-7562 (Print)
  • ISSN: 1469-8102 (Online)

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