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Kinetics, energetics and specificity of a general amino acid transporter from the ectomycorrhizal fungus Paxillus involutus

Författare:
Publiceringsår: 1996
Språk: Engelska
Sidor: 1749-1756
Publikation/Tidskrift/Serie: Microbiology
Volym: 142
Dokumenttyp: Artikel
Förlag: Society for General Microbiology
Övrig information: Ref #19 of 99 in ref_mina.ref

Sammanfattning

The kinetics, energetics and specificity of a general amino acid transporter were studied in the ectomycorrhizal fungus Paxillus involutus (Batsch) Fr. The uptake of amino acids showed features characteristic of active transport. After correction for a non-mediated transport component, the kinetics of glutamate, glutamine, alanine and aspartate uptake measured over a wide concentration range followed the simple Michaelis-Menten saturation curves, The apparent K-m derived from the Eadie-Hofstee plots ranged from 7 mu M for alanine to 27 mu M for glutamate, Dinitrophenol, carbonyl cyanide m-chlorophenylhydrazone and NaN3 strongly inhibited amino acid uptake, whereas dicyclohexylcarbodiimide. vanadate and the ionophores monensin and nonactin had no effect on the uptake. Both ph dependence and inhibition by protonophores are consistent with a proton symport mechanism for amino acid uptake by P. involutus, Competition studies indicated a broad substrate recognition by the uptake system, which resembles the general amino acid permease of yeast, Dixon plots of the inhibition of glutamate uptake by alanine, lysine and methionine sulfoximine showed that inhibitions were competitive, Tire physiological importance of this transporter for the exchange of nitrogenous compounds between fungal and host plant cells in ectomycorrhizal associations is discussed.

Disputation

Nyckelord

  • Biology and Life Sciences
  • amino acid uptake
  • competition and inhibition experiments
  • ectomycorrhizal fungus
  • proton symport mechanism
  • Paxillus involutus

Övrigt

Published
Yes
  • Microbial Ecology
  • ISSN: 1350-0872 (Print)
  • ISSN: 1465-2080 (Online)

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