Respiration of [C-14] alanine by the ectomycorrhizal fungus Paxillus involutus
Författare
Summary, in English
The ectomycorrhizal fungus Paxillus involutus efficiently took up exogenously supplied [C-14]alanine and rapidly converted it to pyruvate, citrate, succinate, fumarate and to CO2, thus providing direct evidence for the utilisation of alanine as a respiratory substrate. [C-14]alanine was further actively metabolised to glutamate, glutamine and aspartate. Exposure to aminooxyacetate completely suppressed (CO2)-C-14 evolution and greatly reduced the flow of carbon from [C-14]alanine to tricarboxylic acid cycle intermediates and amino acids, suggesting that alanine aminotransferase plays a pivotal role in alanine metabolism in Paxillus involutus.
Avdelning/ar
Publiceringsår
1994
Språk
Engelska
Sidor
87-91
Publikation/Tidskrift/Serie
FEMS Microbiology Letters
Volym
121
Issue
1
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
Oxford University Press
Ämne
- Biological Sciences
Nyckelord
- Amino acid respiration
- Aminooxyacetate
- [14C]alanine
- Ectomycorrhizal fungus
- Paxillus involutus
- TCA cycle
Status
Published
Forskningsgrupp
- Microbial Ecology
ISBN/ISSN/Övrigt
- ISSN: 1574-6968