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A new expression vector for production of enzymes in the yeast Saccharomyces (lachancea) kluyveri.

Författare:
Publiceringsår: 2011
Språk: Engelska
Sidor: 1227-1232
Publikation/Tidskrift/Serie: Nucleosides, nucleotides & nucleic acids
Volym: 30
Nummer: 12
Dokumenttyp: Artikel
Förlag: Taylor & Francis Group

Sammanfattning

We overexpressed and purified enzymes involved in the pyrimidine catabolic pathway in the yeast Saccharomyces (Lachancea) kluyveri. A new vector was therefore designed, providing the first specific expression system in Saccharomyces kluyveri. The URC1 gene was overexpressed and a soluble protein obtained and successfully purified using the C-terminally added His-tag. Our system will be used for further studies of the structure and function of the enzymes belonging to the URC pyrimidine degradation pathway.

Disputation

Nyckelord

  • Biology and Life Sciences
  • Expression vector
  • Saccharomyces (Lachancea) kluyveri
  • URC pathway
  • catabolism of pyrimidines

Övriga

Published
Yes
  • ISSN: 1525-7770 (Print)
  • ISSN: 1532-2335 (Online)

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