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Flow-through microdispenser for interfacing mu-HPLC to Raman and mid-IR spectroscopic detection

In English

Författare

Summary, in English

A flow-through microdispenser has been coupled to a micro HPLC separation system and used as a solvent elimination interface for Fourier transform infrared (FTIR) and Raman spectroscopic detection of the separated compounds. Using the microdispenser picoliter sized droplets can be generated and deposited on an appropriate target placed on a computerized x, y-stage. Evaporation of volatile solvent and buffer is rapid and allows analysis of the obtained dry deposits by various techniques. Due to the destruction free character of Raman and FTIR spectroscopy they can be applied sequentially to interrogate the same deposit. In the reported application five phenolic acids typically present in wine have been separated on a C-18 column technique using a mixture of water, methanol and acetic acid as mobile phase. For spectrum acquisition infrared and Raman microscopes have been used. The spectra recorded from the dried deposits of the separated compounds agreed well with the reference spectra of corresponding components.

Publiceringsår

2005

Språk

Engelska

Sidor

132-139

Publikation/Tidskrift/Serie

Journal of Chromatography A

Volym

1080

Issue

2

Dokumenttyp

Artikel i tidskrift

Förlag

Elsevier

Ämne

  • Medical Engineering

Nyckelord

  • flow through
  • HPLC
  • Raman microspectrometry
  • FTIR microscopy
  • microdispenser

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 0021-9673