Du är här

Flow-through microdispenser for interfacing mu-HPLC to Raman and mid-IR spectroscopic detection

Författare:
Publiceringsår: 2005
Språk: Engelska
Sidor: 132-139
Publikation/Tidskrift/Serie: JOURNAL OF CHROMATOGRAPHY A
Volym: 1080
Nummer: 2
Dokumenttyp: Artikel
Förlag: ELSEVIER SCIENCE BV

Sammanfattning

A flow-through microdispenser has been coupled to a micro HPLC separation system and used as a solvent elimination interface for Fourier transform infrared (FTIR) and Raman spectroscopic detection of the separated compounds. Using the microdispenser picoliter sized droplets can be generated and deposited on an appropriate target placed on a computerized x, y-stage. Evaporation of volatile solvent and buffer is rapid and allows analysis of the obtained dry deposits by various techniques. Due to the destruction free character of Raman and FTIR spectroscopy they can be applied sequentially to interrogate the same deposit. In the reported application five phenolic acids typically present in wine have been separated on a C-18 column technique using a mixture of water, methanol and acetic acid as mobile phase. For spectrum acquisition infrared and Raman microscopes have been used. The spectra recorded from the dried deposits of the separated compounds agreed well with the reference spectra of corresponding components.

Disputation

Nyckelord

  • Chemistry
  • flow through
  • HPLC
  • Raman microspectrometry
  • FTIR microscopy
  • microdispenser

Övriga

Published
Yes
  • ISSN: 0021-9673

Box 117, 221 00 LUND
Telefon 046-222 00 00 (växel)
Telefax 046-222 47 20
lu [at] lu [dot] se

LERU logotype U21 logotype

Fakturaadress: Box 188, 221 00 LUND
Organisationsnummer: 202100-3211
Om webbplatsen