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Detection of neurocan in cerebrospinal fluid.

In English

Författare

Summary, in English

Cerebrospinal fluid (CFS) is the most easily accessible component of the human central nervous system and has been successfully used for the analysis of disease-associated molecular imbalances, particularly for extracellular matrix components. Alterations in the presence of the nervous system-associated chondroitin sulfate proteoglycan neurocan had been reported from active multiple sclerosis lesions. Neurocan could be detected as a component of human CFS after enrichment of proteoglycans by anion exchange chromatography from pooled liquor as well as individual 300 μL samples by Western blot. However, a general alteration in neurocan levels in CFS sample with high immunoglobulin content could not be demonstrated. To further reduce the sample size, the development of a PG capturing assay based on polybrene-coated 96-well plates was initiated. This approach could be an interesting alternative option for the analysis of PGs in biological fluid and tissue samples.

Avdelning/ar

Publiceringsår

2012

Språk

Engelska

Sidor

87-95

Publikation/Tidskrift/Serie

Methods in Molecular Biology

Volym

836

Dokumenttyp

Artikel i tidskrift

Förlag

Springer

Ämne

  • Cell and Molecular Biology

Status

Published

Forskningsgrupp

  • Vessel Wall Biology

ISBN/ISSN/Övrigt

  • ISSN: 1940-6029