Du är här

Molecular microheterogeneity of prostate specific antigen in seminal fluid by mass spectrometry.

Publiceringsår: 2012
Språk: Engelska
Sidor: 331-338
Publikation/Tidskrift/Serie: Clinical Biochemistry
Volym: 45
Nummer: 4-5
Dokumenttyp: Artikel
Förlag: Elsevier

Sammanfattning

OBJECTIVES: Prostate specific antigen (PSA) is a widely used and clinically valuable marker for prostate disease. In order to enable the development of new PSA assays and progress the understanding of the biology of PSA we have analyzed PSA in seminal plasma. Design and methods PSA in seminal plasma from men attending a fertility clinic and healthy controls was analyzed using SDS-PAGE, Western blotting and mass spectrometry. RESULTS: Using mass spectrometry, different forms of PSA could be identified in 1-9 bands seen on SDS-PAGE analysis of the respective sample. However, a majority of these molecular forms of PSA were not observed on Western blots. Enzymatic activity of PSA isoforms was demonstrated by sequencing data in zymogram gels. Multivariate analysis of clinical data revealed well-separated patient groups. CONCLUSIONS: We demonstrated that PSA in seminal plasma occurs in several isoforms, yet not all were detectable using an antibody based clinical routine method. The heterogeneity of PSA expression might be of clinical significance, by an improved patient phenotyping.

Disputation

Nyckelord

  • Medicine and Health Sciences
  • ESI-LTQ FT-ICR
  • MALDI LTQ Orbitrap XL
  • infertility
  • seminal plasma
  • prostate specific antigen
  • isoform

Övrigt

Published
Yes
  • ISSN: 1873-2933

Box 117, 221 00 LUND
Telefon 046-222 00 00 (växel)
Telefax 046-222 47 20
lu [at] lu [dot] se

Fakturaadress: Box 188, 221 00 LUND
Organisationsnummer: 202100-3211
Om webbplatsen

LERU logo U21 logo