Du är här

Disposable polymeric high-density nanovial arrays for matrix assisted laser desorption/ionization-time of flight-mass spectrometry: I. Microstructure development and manufacturing

Författare:
Publiceringsår: 2001
Språk: Engelska
Sidor: 3978-3983
Publikation/Tidskrift/Serie: Electrophoresis
Volym: 22
Nummer: 18
Dokumenttyp: Artikel
Förlag: Wiley

Sammanfattning

In order to meet the expected enormous demand for mass spectrometry (MS)through-, put as a result of the current efforts to completely map the human proteome, this paper presents a new concept for low-cost high-throughput protein identification by matrix assisted laser desorption/ionization-time of flight-(MALDI-TOF)-MS peptide mapping using disposable polymeric high-density nanovial MALDI target plates. By means of microfabrication technology precision engineered nanovial arrays are fabricated in polymer substrates such as polymethylmethacrylate (PMMA) and polycarbonate (PC). The target plate fabrication processes investigated were precision. micromilling, cold embossing and injection moulding (work in progress). Nanovial dimensions were 300, 400 or 500 mum. Typical array densities were 165 nanovials/cm(2), which corresponds to 3300 vials on a full Applied Biosystems MALDI target plate. Obtained MALDI data displayed equal mass resolution, accuracy, signal intensity for peptide standards as compared to high-density silicon nanovial arrays previously reported by our group 7, as well as conventional stainless steel or gold targets.

Disputation

Nyckelord

  • Chemistry
  • polymeric high-density matrix-assisted laser desorption
  • ionization
  • target plates
  • nanovial
  • microfabrication
  • peptides
  • array

Övrigt

Published
Yes
  • ISSN: 0173-0835

Box 117, 221 00 LUND
Telefon 046-222 00 00 (växel)
Telefax 046-222 47 20
lu [at] lu [dot] se

Fakturaadress: Box 188, 221 00 LUND
Organisationsnummer: 202100-3211
Om webbplatsen

LERU logo U21 logo