Webbläsaren som du använder stöds inte av denna webbplats. Alla versioner av Internet Explorer stöds inte längre, av oss eller Microsoft (läs mer här: * https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Var god och använd en modern webbläsare för att ta del av denna webbplats, som t.ex. nyaste versioner av Edge, Chrome, Firefox eller Safari osv.

Disposable polymeric high-density nanovial arrays for matrix assisted laser desorption/ionization-time of flight-mass spectrometry: I. Microstructure development and manufacturing

Författare

Summary, in English

In order to meet the expected enormous demand for mass spectrometry (MS)through-, put as a result of the current efforts to completely map the human proteome, this paper presents a new concept for low-cost high-throughput protein identification by matrix assisted laser desorption/ionization-time of flight-(MALDI-TOF)-MS peptide mapping using disposable polymeric high-density nanovial MALDI target plates. By means of microfabrication technology precision engineered nanovial arrays are fabricated in polymer substrates such as polymethylmethacrylate (PMMA) and polycarbonate (PC). The target plate fabrication processes investigated were precision. micromilling, cold embossing and injection moulding (work in progress). Nanovial dimensions were 300, 400 or 500 mum. Typical array densities were 165 nanovials/cm(2), which corresponds to 3300 vials on a full Applied Biosystems MALDI target plate. Obtained MALDI data displayed equal mass resolution, accuracy, signal intensity for peptide standards as compared to high-density silicon nanovial arrays previously reported by our group [7], as well as conventional stainless steel or gold targets.

Publiceringsår

2001

Språk

Engelska

Sidor

3978-3983

Publikation/Tidskrift/Serie

Electrophoresis

Volym

22

Issue

18

Dokumenttyp

Artikel i tidskrift

Förlag

John Wiley & Sons Inc.

Ämne

  • Medical Engineering
  • Analytical Chemistry

Nyckelord

  • polymeric high-density matrix-assisted laser desorption
  • ionization
  • target plates
  • nanovial
  • microfabrication
  • peptides
  • array

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 0173-0835