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Increased beta-cell volume in mice fed a high-fat diet A dynamic study over 12 months

In English

Författare

Summary, in English

As we previously demonstrated, there is an adaptive increase in insulin secretion in insulin resistance in the model of high-fat fed female mice. Since it is assumed that islets also adapt to insulin resistance with beta-cell expansion, we have now examined beta-cell volume in this experimental model. Female C57BL/6JBomTac mice were therefore fed a high-fat diet (60% fat from lard) for three, six or twelve months and beta-cell volume was estimated as beta-cell area per islet, individual beta-cell size and beta-cell number per islet. Control animals were fed a normal chow (11% fat). We found that beta-cell area per islet and total number of beta-cells per islet were increased already after three months of high-fat feeding and that this increase was sustained throughout the twelve month study period. In contrast, individual beta-cell size showed a dynamic pattern with a reduction after three months followed by increase after six and twelve months. The number of apoptosis (caspase-3) positive beta-cells was reduced after three months, whereas there was no difference in proliferation (Ki-67) positive cells, although these were generally rarely observed. Thus, we conclude that insulin resistance accompanying high-fat feeding in mice is followed by progressive beta-cell expansion as evident by early increased islet beta-cell volume and total number of beta-cells, whereas individual beta-cell size showed a dynamic response. The model is also associated with an early reduced apoptosis, which may contribute to the increased beta-cell volume.

Publiceringsår

2010

Språk

Engelska

Sidor

353-356

Publikation/Tidskrift/Serie

Islets

Volym

2

Issue

6

Dokumenttyp

Artikel i tidskrift

Förlag

Landes Bioscience

Ämne

  • Endocrinology and Diabetes

Nyckelord

  • beta cell mass
  • insulin resistance
  • islet adaptation
  • apoptosis
  • proliferation

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1938-2022