IgG glycan hydrolysis by EndoS diminishes the pro-inflammatory properties of immune complexes from patients with SLE - a possible new treatment?
Författare
Summary, in English
METHODS.: ICs, purified from SLE patients or RNA-containing ICs formed in vitro, were treated with EndoS and used in several assays reflecting different important parts of SLE pathogenesis such as phagocytosis by polymorphonuclear neutrophils (PMNs) and plasmacytoid dendritic cells (pDCs), complement activation and IFNα production by pDCs.
RESULTS.: Our results demonstrate that EndoS treatment could abolish all pro-inflammatory properties of ICs investigated. This includes FcγR-mediated phagocytosis by pDCs (p<0.0001) and subsequent production of IFNα (p<0.0001), IC-induced classical complement pathway activation (p<0.0001), chemotaxis and oxidative burst activity of PMNs (p=0.002). We could also demonstrate direct effects on the molecular structure of ICs after EndoS treatment with decreased size and glycosylation patterns.
CONCLUSIONS.: Prominent effects of EndoS treatment were seen in several pathogenetically important IC-mediated events and our data suggest that EndoS have the potential to be developed as a novel therapy in SLE. © 2012 American College of Rheumatology.
Avdelning/ar
Publiceringsår
2012
Språk
Engelska
Publikation/Tidskrift/Serie
Arthritis and Rheumatism
Volym
71
Issue
Suppl 1
Länkar
Dokumenttyp
Konferensbidrag: abstract
Förlag
John Wiley & Sons Inc.
Ämne
- Rheumatology and Autoimmunity
Conference name
32nd European Workshop for Rheumatology Research
Conference date
2012-02-23 - 2012-02-25
Conference place
Stockholm, Sweden
Status
Published
Forskningsgrupp
- Infection and immunomodulation
ISBN/ISSN/Övrigt
- ISSN: 1529-0131