Du är här

Deoxyribonucleoside kinases in two aquatic bacteria with high specificity for thymidine and deoxyadenosine.

Författare:
Publiceringsår: 2012
Språk: Engelska
Sidor: 120-127
Publikation/Tidskrift/Serie: FEMS microbiology letters
Volym: 331
Nummer: 2
Dokumenttyp: Artikel
Förlag: Wiley-Blackwell Publishing, Inc

Sammanfattning

Deoxyribonucleoside kinases (dNKs) are essential in the mammalian cell but their 'importance' in bacteria, especially aquatic ones, is less clear. We studied two aquatic bacteria, Gram-negative Flavobacterium psychrophilum JIP02/86 and Polaribacter sp. MED152, for their ability to salvage deoxyribonucleosides (dNs). Both had a Gram-positive-type thymidine kinase (TK1), which could phosphorylate thymidine, and one non-TK1 dNK, which could efficiently phosphorylate deoxyadenosine and slightly also deoxycytosine. Surprisingly, the four tested dNKs could not phosphorylate deoxyguanosine, and apparently, these two bacteria are missing this activity. When tens of available aquatic bacteria genomes were examined for the presence of dNKs, a majority had at least a TK1-like gene, but several lacked any dNKs. Apparently, among aquatic bacteria, the role of the dN salvage varies.

Disputation

Nyckelord

  • Biology and Life Sciences
  • aquatic bacteria
  • 3H-thymidine incorporation
  • deoxyribonucleoside kinase
  • nucleoside salvage

Övrigt

Published
Yes
  • ISSN: 1574-6968 (Online)
  • ISSN: 0378-1097 (Print)

Box 117, 221 00 LUND
Telefon 046-222 00 00 (växel)
Telefax 046-222 47 20
lu [at] lu [dot] se

Fakturaadress: Box 188, 221 00 LUND
Organisationsnummer: 202100-3211
Om webbplatsen

LERU logo U21 logo