Autonomous protein sample processing on-chip using solid-phase microextraction, capillary force pumping, and microdispensing
Författare
Summary, in English
A capillary force filling microsystem consisting of a chip-integrated solid-phase microextraction (SMEC) array and a microdispenser for sample purification and trace enrichment of peptides is described. The microextraction array was loaded with solid-phase media (50 mum Poros R2 beads) for purification and enrichment of proteomic samples. Samples bound to the SMEC were eluted in a volume of 200 nL. A piezoelectric microdispenser was docked to the array and the samples bound to the SMEC were eluted in a volume of 200 nL using capillary forces. The purified and enriched samples were dispensed onto the matrix-assisted laser desorption/ionization (MALDI) target, providing quality data from samples in the picomolar range. The nanoproteomic platform was compared to corresponding commercial preparation protocols, showing higher mass spectrometry (MS) signal intensities for peptides generated from an alpha-casein digest. The platform was also elvaluated with regards to two-dimensional (2-D) gel-derived protein digests from both fibroblast and epithelial target cells.
Publiceringsår
2004
Språk
Engelska
Sidor
3778-3787
Publikation/Tidskrift/Serie
Electrophoresis
Volym
25
Issue
21-22
Dokumenttyp
Artikel i tidskrift
Förlag
John Wiley & Sons Inc.
Ämne
- Medical Engineering
- Analytical Chemistry
Nyckelord
- microextraction
- miniaturization
- mass spectrometry
- solid-phase
- proteomics
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 0173-0835