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Autonomous protein sample processing on-chip using solid-phase microextraction, capillary force pumping, and microdispensing

Författare

Summary, in English

A capillary force filling microsystem consisting of a chip-integrated solid-phase microextraction (SMEC) array and a microdispenser for sample purification and trace enrichment of peptides is described. The microextraction array was loaded with solid-phase media (50 mum Poros R2 beads) for purification and enrichment of proteomic samples. Samples bound to the SMEC were eluted in a volume of 200 nL. A piezoelectric microdispenser was docked to the array and the samples bound to the SMEC were eluted in a volume of 200 nL using capillary forces. The purified and enriched samples were dispensed onto the matrix-assisted laser desorption/ionization (MALDI) target, providing quality data from samples in the picomolar range. The nanoproteomic platform was compared to corresponding commercial preparation protocols, showing higher mass spectrometry (MS) signal intensities for peptides generated from an alpha-casein digest. The platform was also elvaluated with regards to two-dimensional (2-D) gel-derived protein digests from both fibroblast and epithelial target cells.

Publiceringsår

2004

Språk

Engelska

Sidor

3778-3787

Publikation/Tidskrift/Serie

Electrophoresis

Volym

25

Issue

21-22

Dokumenttyp

Artikel i tidskrift

Förlag

John Wiley & Sons Inc.

Ämne

  • Medical Engineering
  • Analytical Chemistry

Nyckelord

  • microextraction
  • miniaturization
  • mass spectrometry
  • solid-phase
  • proteomics

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 0173-0835