Meny

Du är här

Exploring the pharmacology of the leukotriene B-4 receptor BLT1, without the confounding effects of BLT2

Författare:
Publiceringsår: 2004
Språk: Engelska
Sidor: 53-65
Publikation/Tidskrift/Serie: European Journal of Pharmacology
Volym: 499
Nummer: 1-2
Dokumenttyp: Artikel
Förlag: Elsevier Science B.V.

Sammanfattning

Most previous studies of leukotriene B-4 (LTB4) pharmacology using primary leukocyte cultures and myeloid cell lines do not differentiate between leukotriene BLT1 and BLT1 receptor activation because both receptors are often expressed by these cells. Here we show that in HeLa cells expressing BLT1 but not BLT2 receptors, BLT1 receptor activation resulted in IP3 mediated calcium release from intracellular stores initially, followed by calcium influx through cell membrane channels. BLT1 calcium signalling was sensitive to the activity of protein kinase C (PKC). protein kinase A (PKA) and protein-tyrosine kinases (PTKs), as well as changes in membrane cholesterol levels and treatments that are known to disrupt normal membrane physiology and/or lipid rafts. Inhibition of MAP kinases, Rho-associated kinases, or phosphomositol-3-kinases (PI3K) had no effect on BLT1 receptor induced calcium signalling, and the receptor was insensitive to the redox state of the extracellular compartment. (C) 2004 Elsevier B.V. All rights reserved.

Disputation

Nyckelord

  • Medicine and Health Sciences
  • fura-2
  • BLT1
  • leukotriene B-4
  • g-protein coupled receptor
  • pharmacology
  • calcium

Övriga

Published
Yes
  • ISSN: 0014-2999

Box 117, 221 00 LUND
Telefon 046-222 00 00 (växel)
Telefax 046-222 47 20
lu [at] lu [dot] se

Fakturaadress: Box 188, 221 00 LUND
Organisationsnummer: 202100-3211
Om webbplatsen