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Canavanine content of sword beans (Canavalia gladiata) – analysis and effect of processing

In English

Författare

  • Sagarika Ekanayake
  • Kerstin Skog
  • Nils-Georg Asp

Summary, in English

The amino acid canavanine is a potentially toxic constituent of leguminous seeds. The aim of the present study was to determine the ability of different processing methods to reduce canavanine in sword beans (Canavalia gladiata). For this purpose a method for the detection and quantification of canavanine was developed using reversed-phase high-performance liquid chromatography of the dabsylated derivatives. The recovery of canavanine using this method was 88–91%. Optimum extraction of canavanine from raw and processed beans was obtained by addition of hot water prior to overnight soaking. The results obtained with this method agree well with previously published values for raw seeds. The method is sensitive, specific and can successfully be applied to the detection of canavanine in legumes.



Overnight soaking and boiling in excess water followed by decanting gave the most pronounced reduction in canavanine content (around 50%), followed by boiling and decanting excess water (34%). Roasting as used in this study and autoclaving were less effective in reducing the canavanine content.

Publiceringsår

2007

Språk

Engelska

Sidor

797-803

Publikation/Tidskrift/Serie

Food and Chemical Toxicology

Volym

45

Issue

5

Dokumenttyp

Artikel i tidskrift

Förlag

Elsevier

Ämne

  • Pharmacology and Toxicology

Nyckelord

  • Sword beans
  • HPLC
  • Dabsyl derivative
  • Canavanine
  • Processing

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 0278-6915