Meny

Javascript is not activated in your browser. This website needs javascript activated to work properly.
Du är här

Automated multi-dimensional liquid chromatography: sample preparation and identification of peptides from human blood filtrate

Författare:
Publiceringsår: 2004
Språk: Engelska
Sidor: 121-130
Publikation/Tidskrift/Serie: Journal of Chromatography B
Volym: 803
Nummer: 1
Dokumenttyp: Artikel
Förlag: Elsevier

Sammanfattning

A comprehensive on-line sample clean-up with an integrated two-dimensional HPLC system was developed for the analysis of natural peptides. Samples comprised of endogenous peptides with molecular weights up to 20 kDa were generated from human hemofiltrate (HF) obtained from patients with chronic renal failure. The (poly-)peptides were separated using novel silica-based restricted access materials with strong cation-exchange functionalities (SCX-RAM). The size-selective sample fractionation step is followed by cation-exchange chromatography as the first dimension. The subsequent second dimension of separation is based on hydrophobic interaction using four parallel short reversed-phase (RP) columns implemented via a fully automated column switching technique. More than 1000 peaks were resolved within the total analysis time of 96 min. Substances of selected peaks were sampled to analyse their molecular weights by off-line MALDI-TOF mass spectrometry and to determine their amino acid sequence by Edman degradation. The potential for comprehensive peptide mapping and identification is demonstrated.

Disputation

Nyckelord

  • Chemistry
  • sample preparation
  • identification
  • human blood filtrate
  • peptides

Övriga

Published
Yes
  • ISSN: 1570-0232

Box 117, 221 00 LUND
Telefon 046-222 00 00 (växel)
Telefax 046-222 47 20
lu [at] lu [dot] se

Fakturaadress: Box 188, 221 00 LUND
Organisationsnummer: 202100-3211
Om webbplatsen