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Isolation, characterization, and enzymatic hydrolysis of acetyl-galactoglucomannan from spruce (Picea abies)

Författare:
Publiceringsår: 2004
Språk: Engelska
Sidor: 66-78
Publikation/Tidskrift/Serie: Hemicelluloses. Science and Technology (ACS Symposium Series)
Volym: 864
Dokumenttyp: Del av eller Kapitel i bok
Förlag: American Chemical Society Publication

Sammanfattning

Water-soluble hemicelluloses were extracted from spruce chips by heat-fractionation using microwave treatment. A screening of conditions (pH, temperature and residence time) was performed for the extraction of O-acetyl-galactoglucomannan (AcGGM). The yield and the average molecular weight of the extracted mannan were analysed using HPLC, size-exclusion chromatography (SEC) and mass-spectrometry. The pH during heat fractionation influenced the yield and the structure of AcGGM. The highest yield (78%) of AcGGM (average molecular weight 3800) was achieved with heat-fractionation in water at 190degrees C for 5 minutes. With 0.025% NaOH, an average molecular weight of 9500 was obtained at a yield of 30%. AcGGM molecular weight standard molecules were prepared using SEC. The structure of the AcGGM was determined using H-1-NMR. The enzymatic hydrolysis of AcGGM was studied using beta-mannanase and alpha-galactosidase.

Disputation

Nyckelord

  • Chemistry
  • DEGRADING ENZYMES
  • MICROWAVE IRRADIATION
  • BETA-MANNANASE
  • HEMICELLULOSES
  • PRETREATMENT
  • CHROMATOGRAPHY
  • LIGNOCELLULOSE
  • GLUCOMANNAN
  • POSITIONS
  • PINUS-SILVESTRIS L

Övriga

Published
  • ISSN: 0097-6156
  • ISBN: 978-0-8412-3842-8
  • ISBN: 0-8412-3842-1

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