Epithelial G protein-coupled receptor kinases regulate the initial inflammatory response during mycobacterial infection.
Författare
Summary, in English
The interaction between mycobacteria and epithelium is unexplored, but may determine the outcome of the infection. We have analyzed the role of two G protein-coupled receptors, CXCR1 and CXCR2 that are important regulators of many pulmonary diseases. We found that mycobacteria significantly increased the expression of both CXCR1 and CXCR2 on alveolar epithelial cells and both receptors were found to be important for neutrophil diapedesis across primary endothelial cells towards infected mucosa. Mycobacteria, lipoarabinomannan or 19-kDa glycolipoprotein up-regulated the inhibitory G protein-coupled receptor kinase (GRK)2, while GRK3 was less affected. Mycobacteria-induced GRK2 up-regulation decreased chemokine transcription and secretion thereby affecting the neutrophil recruitment to infected mucosa. These events were completely abolished by blocking these receptors prior to infection as the blocking increased epithelial immune responses. We have identified novel interactions occurring in the initial phase of mycobacterial infections by which mycobacterial manipulate epithelial inflammatory responses.
Avdelning/ar
Publiceringsår
2013
Språk
Engelska
Sidor
984-994
Publikation/Tidskrift/Serie
Immunobiology
Volym
218
Issue
7
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
Elsevier
Ämne
- Immunology in the medical area
Status
Published
Forskningsgrupp
- Lung Biology
ISBN/ISSN/Övrigt
- ISSN: 1878-3279