Degradation of Pyrimidines in Saccharomyces Kluyveri: Transamination of beta-Alanine.

Summary, in English

Beta-alanine is an intermediate in the reductive degradation of uracil. Recently we have identified and characterized the Saccharomyces kluyveri PYD4 gene and the corresponding enzyme beta -alanine aminotransferase ((Sk)Pyd4p), highly homologous to eukaryotic gamma-aminobutyrate aminotransferase (GABA-AT). S. kluyveri has two aminotransferases, GABA aminotransferase ((Sk)Uga1p) with 80% and (Sk)Pyd4p with 55% identity to S. cerevisiae GABA-AT. (Sk)Pyd4p is a typical pyridoxal phosphate-dependent aminotransferase, specific for alpha -ketoglutarate (alpha KG), beta -alanine (BAL) and gamma -aminobutyrate (GABA), showing a ping-pong kinetic mechanism involving two half-reactions and substrate inhibition. (Sk)Uga1p accepts only alpha KG and GABA but not BAL, thus only (Sk)Pydy4p belongs to the uracil degradative pathway.

• Författare: K D Schnackerz; Gorm Andersen; D Dobritzsch; Jure Piskur
• Avdelning/ar: Biologiska institutionen; Molekylär cellbiologi
• Publiceringsår: 2008
• Språk: Engelska
• Sidor: 794-799
• Publikation/Tidskrift/Serie: Nucleosides, Nucleotides & Nucleic Acids
• Volym: 27
• Issue: 6
• Länkar: http://dx.doi.org/10.1080/15257770802145983
• Dokumenttyp: Artikel i tidskrift
• Förlag: Taylor & Francis
• Ämne: Biological Sciences
• Nyckelord: Saccharomyces kluyveri; Aminotransferase; gene duplication; γ-aminobutyrate; β-alanine
• Status: Published
• ISBN/ISSN/Övrigt: ISSN: 1525-7770