Improved porous silicon microarray based prostate specific antigen immunoassay by optimized surface density of the capture antibody
Författare
Summary, in English
Enriching the surface density of immobilized capture antibodies enhances the detection signal of antibody sandwich microarrays. In this study, we improved the detection sensitivity of our previously developed P-Si (porous silicon) antibody microarray by optimizing concentrations of the capturing antibody. We investigated immunoassays using a P-Si microarray at three different capture antibody (PSA - prostate specific antigen) concentrations, analyzing the influence of the antibody density on the assay detection sensitivity. The LOD (limit of detection) for PSA was 2.5 ng mL(-1), 80 pg mL(1), and 800 fg mL(-1) when arraying the PSA antibody, H117 at the concentration 15 mu g mL(-1), 35 mu g mL(-1), and 154 mu g mL(-1), respectively. We further investigated PSA spiked into human female serum in the range of 800 fg mL(-1) to 500 ng mL(-1). The microarray showed a LOD of 800 fg mL(-1) and a dynamic range of 800 fg mL(-1) to 80 ng mL(-1) in serum spiked samples. (C) 2013 Elsevier B.V. All rights reserved.
Avdelning/ar
- Klinisk kemi, Malmö
- Avdelningen för Biomedicinsk teknik
- EpiHealth: Epidemiology for Health
- MultiPark: Multidisciplinary research focused on Parkinson´s disease
- BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation
Publiceringsår
2013
Språk
Engelska
Sidor
108-114
Publikation/Tidskrift/Serie
Analytica Chimica Acta
Volym
796
Dokumenttyp
Artikel i tidskrift
Förlag
Elsevier
Ämne
- Analytical Chemistry
Nyckelord
- Antibody microarray
- Porous silicon
- Sandwich immunoassay
- Prostate
- specific antigen
Status
Published
Forskningsgrupp
- Clinical Chemistry, Malmö
ISBN/ISSN/Övrigt
- ISSN: 1873-4324